Lane 1: Lane 2:
BBM1+2+3 BBM3+4+5
BBF1+2+3 BBF3+4+5
BSM1+2+3 BSM3+4+5
BSF1+2+3 BSF3+4+5
SBM1+2+3 SBM3+4+5
SBF1+2+3 SBF3+4+5
SSM1+2+3 SSM3+4+5
SSF1+2+3 SSF3+4+5
Each of these numbers will have it's own unique barcode. But as I only have 24 barcodes, some will have to be repeated between the lanes. For example, BBM3 must go in both lanes and will have the same barcode. This will force BBM1 and BBM4 to share a barcode, same with BBM2 and BBM5. It will end up with barcodes 1-16 in each lane but on different samples, and barcodes 17-24 in each lane and on the same sample.
Here is a schematic of my plan:
Here is a schematic of my plan:
Sample ID
|
Illumina Barcode Number
|
Reference Number (above)
|
Lane
| |
1
|
BB.BB15.3M
|
1
|
BBM1
|
1
|
2
|
BB.SS70.3M
|
2
|
BSM1
|
1
|
3
|
SS.BB20.2M
|
3
|
SBM1
|
1
|
4
|
SS.SS82.1M
|
4
|
SSM1
|
1
|
5
|
BB.BB15.4F
|
5
|
BBF1
|
1
|
6
|
BB.SS70.2F
|
6
|
BSF1
|
1
|
7
|
SS.BB20.6F
|
7
|
SBF1
|
1
|
8
|
SS.SS82.2F
|
8
|
SSF1
|
1
|
9
|
BB.BB77.2M
|
9
|
BBM2
|
1
|
10
|
BB.SS70.5M
|
10
|
BSM2
|
1
|
11
|
SS.BB25.5M
|
11
|
SBM2
|
1
|
12
|
SS.SS87.1M
|
12
|
SSM2
|
1
|
13
|
BB.BB77.1F
|
13
|
BBF2
|
1
|
14
|
BB.SS70.4F
|
14
|
BSF2
|
1
|
15
|
SS.BB25.3F
|
15
|
SBF2
|
1
|
16
|
SS.SS87.3F
|
16
|
SSF2
|
1
|
17
|
BB.BB86.2M
|
17
|
BBM3
|
both
|
18
|
BB.SS72.2M
|
18
|
BSM3
|
both
|
19
|
SS.BB25.3M
|
19
|
SBM3
|
both
|
20
|
SS.SS88.1M
|
20
|
SSM3
|
both
|
21
|
BB.BB86.1F
|
21
|
BBF3
|
both
|
22
|
BB.SS72.1F
|
22
|
BSF3
|
both
|
23
|
SS.BB29.1F
|
23
|
SBF3
|
both
|
24
|
SS.SS88.3F
|
24
|
SSF3
|
both
|
25
|
BB.BB87.6M
|
1
|
BBM4
|
2
|
26
|
BB.SS73.3M
|
2
|
BSM4
|
2
|
27
|
SS.BB20.8M
|
3
|
SBM4
|
2
|
28
|
SS.SS89.2M
|
4
|
SSM4
|
2
|
29
|
BB.BB87.1F
|
5
|
BBF4
|
2
|
30
|
BB.SS73.1F
|
6
|
BSF4
|
2
|
31
|
SS.BB24.2F
|
7
|
SBF4
|
2
|
32
|
SS.SS89.1F
|
8
|
SSF4
|
2
|
33
|
BB.BB77.3M
|
9
|
BBM5
|
2
|
34
|
BB.SS71.3M
|
10
|
BSM5
|
2
|
35
|
SS.BB22.4M
|
11
|
SBM5
|
2
|
36
|
SS.SS91.1M
|
12
|
SSM5
|
2
|
37
|
BB.BB90.1F
|
13
|
BBF5
|
2
|
38
|
BB.SS73.2F
|
14
|
BSF5
|
2
|
39
|
SS.BB24.3F
|
15
|
SBF5
|
2
|
40
|
SS.SS91.4F
|
16
|
SSF5
|
2
|
Furthermore, I will need to prepare these in even batches to avoid any weird batch effects. I will therefore do them in 5 batches of 8. Each batch will have one male and one female from each cross type.
I'll start the first batch (numbers 1-8) tomorrow.
I'll start the first batch (numbers 1-8) tomorrow.
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