#
|
Date
extracted
|
ID
|
RIN
|
[ ] ng/uL
|
Library
|
1
|
9-28-12
|
BB.BB15.3M
|
9.6
|
1071
|
Yes
|
2
|
9-28-12
|
BB.SS70.3M
|
9.6
|
1127
|
Yes
|
3
|
9-28-12
|
SS.BB20.2M
|
9.7
|
859
|
Yes
|
4
|
9-28-12
|
SS.SS82.1M
|
9.8
|
1543
|
Yes
|
5
|
9-28-12
|
BB.BB15.4F
|
9.4
|
1690
|
Yes
|
6
|
9-28-12
|
BB.SS70.2F
|
9.4
|
1427
|
Yes
|
7
|
9-28-12
|
SS.BB20.6F
|
9.6
|
1818
|
Yes
|
8
|
9-28-12
|
SS.SS82.2F
|
9.6
|
1808
|
Yes
|
9
|
9-29-12
|
BB.BB77.2M
|
9.3
|
3086
|
Yes
|
10
|
9-29-12
|
BB.SS70.5M
|
9.4
|
5631
|
Yes
|
11
|
9-29-12
|
SS.BB25.5M
|
9.7
|
1783
|
Yes
|
12
|
9-29-12
|
SS.SS87.1M
|
9.5
|
2363
|
Yes
|
13
|
12-15-12
|
BB.BB77.1F
|
9.4
|
512
|
Yes
|
14
|
12-15-12
|
BB.SS70.4F
|
9.3
|
367
|
Yes
|
15
|
12-15-12
|
SS.BB25.3F
|
9.4
|
553
|
Yes
|
16
|
12-15-12
|
SS.SS87.3F
|
9.6
|
455
|
Yes
|
17
|
12-15-12
| BB.BB86.2M | 9.4 | 449 |
Yes
|
18
|
12-15-12
|
BB.SS72.2M
|
9.6
|
483
|
Yes
|
19
|
12-15-12
|
SS.BB25.3M
|
9.3
|
326
|
Yes
|
20
|
12-15-12
|
SS.SS88.1M
|
9.6
|
440
|
Yes
|
21
|
12-15-12
|
BB.BB86.1F
| 9.1 | 235 |
Yes
|
22
|
12-15-12
|
BB.SS72.1F
|
8.9
|
354
|
Yes
|
23
|
12-15-12
|
SS.BB29.1F
|
9.1
|
266
|
Yes
|
24
|
12-15-12
|
SS.SS88.3F
|
9.5
|
507
|
Yes
|
25
|
12-17-12
|
BB.BB87.6M
|
9.1
|
466
|
Yes
|
26
|
12-17-12
|
BB.SS73.3M
|
8.6*
|
466
|
Yes
|
27
|
12-17-12
|
SS.BB20.8M
|
9.0
|
354
|
Yes
|
28
|
12-17-12
|
SS.SS89.2M
| 9.0 |
254
|
Yes
|
29
|
12-17-12
|
BB.BB87.1F
|
9.0
|
411
|
Yes
|
30
|
12-17-12
|
BB.SS73.1F
|
9.1
|
414
|
Yes
|
31
|
12-17-12
|
SS.BB24.2F
|
8.7
|
257
|
Yes
|
32
|
12-17-12
|
SS.SS89.1F
|
9.0
|
394
|
Yes
|
33
|
2-19-13
|
BB.BB77.3M
|
9.1
| 519 |
Yes
|
34
|
2-19-13
|
BB.SS71.3M
|
8.4
|
284
|
Yes
|
35
|
2-19-13
|
SS.BB22.4
|
8.5
|
273
|
Yes
|
36
| 2-19-13 |
SS.SS91.1M
|
9.5
|
557
|
Yes
|
37
|
2-19-13
|
BB.BB90.1F
|
8.3
|
448
|
Yes
|
38
|
2-19-13
|
BB.SS73.2F
|
8.4
|
318
|
Yes
|
39
|
2-19-13
|
SS.BB24.3F
|
8.1
|
281
|
Yes
|
40
|
2-19-13
|
SS.SS91.4F
|
8.5
|
563
|
Yes
|
12-17-12: I was going to try to finish everything up today or tomorrow, but I ran out of reagents. I ordered more but they won't get in for a little while. I'm really frustrated as I was hoping to finish everything up before I head home for the break. Now it looks like it won't get finished until February.
12-18-12: It's good that I didn't extract the other 8 yesterday, as the RIN numbers were all quite low. I'm not sure why, but I need to get that straightened out. I got one of the reagents in the mail today, now I'm just waiting on the other and I will be set to finish everything off.
2-20-13: Just bioanalyzed the last samples. Not great, but D~ says they are good enough - Illumina calls for >8. J~ was fine with it since there are only 8/40 that are between 8 and 9.
*There is one sample that was only 7.4 (BB.SS73.3M) and I re-bioanalyzed it today it went down to 5.6. This says to me that it has some RNase contaminant in it and is being actively degraded. I have some RNA partially extracted and archived that I will finish extracting, but most likely I will need to find a new sample. Though, having 1 out of 40 go bad is fine by me, that's pretty good odds.
*4-1-13 Upon re-extracting this sample the RIN was 8.6 and the concentration was a bit high (so the actual RIN is likely much higher) I can now finish up with the library preps.
12-18-12: It's good that I didn't extract the other 8 yesterday, as the RIN numbers were all quite low. I'm not sure why, but I need to get that straightened out. I got one of the reagents in the mail today, now I'm just waiting on the other and I will be set to finish everything off.
2-20-13: Just bioanalyzed the last samples. Not great, but D~ says they are good enough - Illumina calls for >8. J~ was fine with it since there are only 8/40 that are between 8 and 9.
*There is one sample that was only 7.4 (BB.SS73.3M) and I re-bioanalyzed it today it went down to 5.6. This says to me that it has some RNase contaminant in it and is being actively degraded. I have some RNA partially extracted and archived that I will finish extracting, but most likely I will need to find a new sample. Though, having 1 out of 40 go bad is fine by me, that's pretty good odds.
*4-1-13 Upon re-extracting this sample the RIN was 8.6 and the concentration was a bit high (so the actual RIN is likely much higher) I can now finish up with the library preps.
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